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trcn0000416347  (Addgene inc)


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    Structured Review

    Addgene inc trcn0000416347
    Trcn0000416347, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plko+1+shjund+puro/tet-pLKO%2E1_puro_shJUND+%231+(Plasmid+%23136581)/pmc07315801-778-37-38
    Average 91 stars, based on 1 article reviews
    trcn0000416347 - by Bioz Stars, 2026-09
    91/100 stars

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    Related Articles

    Construct:

    Article Title: A time- and matrix-dependent TGFBR3–JUND–KRT5 regulatory circuit in single breast epithelial cells and basal-like premalignancies
    Article Snippet: MDA-MB-468 cells were obtained from ATCC and cultured in L-15 medium (Invitrogen) plus 10% fetal bovine serum (Hyclone) without supplemental CO 2 . .. pLKO.1 shGFP puro (Addgene #12273), pLKO.1 shTGFBR3 puro (TRCN0000033430), and pLKO.1 shJUND puro (TRCN0000014974) were obtained from The RNAi Consortium or Addgene. pBabe JunD-HA neo, pBabe JUND-HA neo, pBabe RFP1-Smad2 neo, pBabe JunD-Venus puro, and pBabe RFP1-KRT5 hygro were constructed by PCR cloning from plasmid templates (Open Biosystems) into the retroviral vector pBabe neo, pBabe puro, or pBabe hygro. ..

    Polymerase Chain Reaction:

    Article Title: A time- and matrix-dependent TGFBR3–JUND–KRT5 regulatory circuit in single breast epithelial cells and basal-like premalignancies
    Article Snippet: MDA-MB-468 cells were obtained from ATCC and cultured in L-15 medium (Invitrogen) plus 10% fetal bovine serum (Hyclone) without supplemental CO 2 . .. pLKO.1 shGFP puro (Addgene #12273), pLKO.1 shTGFBR3 puro (TRCN0000033430), and pLKO.1 shJUND puro (TRCN0000014974) were obtained from The RNAi Consortium or Addgene. pBabe JunD-HA neo, pBabe JUND-HA neo, pBabe RFP1-Smad2 neo, pBabe JunD-Venus puro, and pBabe RFP1-KRT5 hygro were constructed by PCR cloning from plasmid templates (Open Biosystems) into the retroviral vector pBabe neo, pBabe puro, or pBabe hygro. ..

    Cloning:

    Article Title: A time- and matrix-dependent TGFBR3–JUND–KRT5 regulatory circuit in single breast epithelial cells and basal-like premalignancies
    Article Snippet: MDA-MB-468 cells were obtained from ATCC and cultured in L-15 medium (Invitrogen) plus 10% fetal bovine serum (Hyclone) without supplemental CO 2 . .. pLKO.1 shGFP puro (Addgene #12273), pLKO.1 shTGFBR3 puro (TRCN0000033430), and pLKO.1 shJUND puro (TRCN0000014974) were obtained from The RNAi Consortium or Addgene. pBabe JunD-HA neo, pBabe JUND-HA neo, pBabe RFP1-Smad2 neo, pBabe JunD-Venus puro, and pBabe RFP1-KRT5 hygro were constructed by PCR cloning from plasmid templates (Open Biosystems) into the retroviral vector pBabe neo, pBabe puro, or pBabe hygro. ..

    Plasmid Preparation:

    Article Title: A time- and matrix-dependent TGFBR3–JUND–KRT5 regulatory circuit in single breast epithelial cells and basal-like premalignancies
    Article Snippet: MDA-MB-468 cells were obtained from ATCC and cultured in L-15 medium (Invitrogen) plus 10% fetal bovine serum (Hyclone) without supplemental CO 2 . .. pLKO.1 shGFP puro (Addgene #12273), pLKO.1 shTGFBR3 puro (TRCN0000033430), and pLKO.1 shJUND puro (TRCN0000014974) were obtained from The RNAi Consortium or Addgene. pBabe JunD-HA neo, pBabe JUND-HA neo, pBabe RFP1-Smad2 neo, pBabe JunD-Venus puro, and pBabe RFP1-KRT5 hygro were constructed by PCR cloning from plasmid templates (Open Biosystems) into the retroviral vector pBabe neo, pBabe puro, or pBabe hygro. ..

    Retroviral:

    Article Title: A time- and matrix-dependent TGFBR3–JUND–KRT5 regulatory circuit in single breast epithelial cells and basal-like premalignancies
    Article Snippet: MDA-MB-468 cells were obtained from ATCC and cultured in L-15 medium (Invitrogen) plus 10% fetal bovine serum (Hyclone) without supplemental CO 2 . .. pLKO.1 shGFP puro (Addgene #12273), pLKO.1 shTGFBR3 puro (TRCN0000033430), and pLKO.1 shJUND puro (TRCN0000014974) were obtained from The RNAi Consortium or Addgene. pBabe JunD-HA neo, pBabe JUND-HA neo, pBabe RFP1-Smad2 neo, pBabe JunD-Venus puro, and pBabe RFP1-KRT5 hygro were constructed by PCR cloning from plasmid templates (Open Biosystems) into the retroviral vector pBabe neo, pBabe puro, or pBabe hygro. ..



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